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Fluorescence-lifetime imaging microscopy or FLIM is an imaging technique for producing an image based on the differences in the exponential decay rate of the fluorescence from a fluorescent sample. It can be used as an imaging technique in confocal microscopy, two-photon excitation microscopy, and multiphoton tomography. The lifetime of the fluorophore signal, rather than its intensity, is used to create the image in FLIM. This has the advantage of minimizing the effect of photon scattering in thick layers of sample. ==Fluorescence lifetimes== A fluorophore which is excited by a photon will drop to the ground state with a certain probability based on the decay rates through a number of different (radiative and/or nonradiative) decay pathways. To observe fluorescence, one of these pathways must be by spontaneous emission of a photon. In the ensemble description, the fluorescence emitted will decay with time according to : where :. In the above, is time, is the fluorescence lifetime, is the initial fluorescence at , and are the rates for each decay pathway, at least one of which must be the fluorescence decay rate . More importantly, the lifetime, is independent of the initial intensity and of the emitted light. This can be utilized for making non-intensity based measurements in chemical sensing.〔Joseph R. Lakowicz. (Principles of Fluorescence Spectroscopy ) 3rd edition. Springer (2006). ISBN 978-0387-31278-1.〕 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Fluorescence-lifetime imaging microscopy」の詳細全文を読む スポンサード リンク
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